Geochemical investigation of the mixed Máriahalom vertebrate fauna at the Paleogene–Neogene boundary in the Central Paratethys: environmental conditions and age constrain
Swiss Journal of Palaeontology volume 142, Article number: 17 (2023)
The fossil vertebrate fauna of Máriahalom contains remains from a wide range of ecologies including terrestrial and aquatic mammals, crocodiles, sharks, and rays among others. All these were found mixed in mollusc-rich, shallow water, coastal deposits. The aim of the study is to trace the origin of the fossils using their rare earth element (REE) content and their respective ecology with stable oxygen isotopic compositions. In addition, marine vertebrates and calcareous marine fossils were analysed for their Sr isotope composition to provide a new age estimate for the locality. The REE content and their distribution in the fossils indicate similar early diagenetic environments and possible contemporaneous fossilization for the entire vertebrate assemblage. Reworked fossils of significantly different age can be excluded. The enamel/enameloid-derived phosphate oxygen isotope composition of selected fossil taxa fit well with previously inferred habitats that include marine, brackish, and terrestrial environments. Notably, the stem-pinniped Potamotherium valletoni is best interpreted as freshwater dweller instead of marine, consistent with the sedimentology of other occurrences. Our novel 87Sr/86Sr data suggest an Aquitanian age (21.4 ± 0.5 Ma) for the Máriahalom site that is younger than the previously proposed Late Oligocene age based on biostratigraphy (MP28–30 European Mammal Paleogene Reference Levels). An Aquitanian age raises the possibility that the index fossil taxon, the anthracothere mammal Microbunodon minimum, may have vanished earlier in Western Europe than in the Central Paratethys region.
The fossil locality of Máriahalom (Mány-Zsambék Basin, northern Hungary) yielded a rich mollusc assemblage as well as a mixed vertebrate fauna of marine, freshwater, and terrestrial origins, deposited in a shallow marine coastal setting influenced by tide and wave actions of the Central Paratethys (Fig. 1) (Báldi, 1967; Báldi & Cságoly, 1975; Sztanó et al., 1998; Rabi & Botfalvai, 2008; Szabó et al., 2017; Rabi et al., 2018). The age of the beds is considered Egerian (Late Chattian—Early Aquitanian), but the exact boundary of the Paleogene and Neogene cannot be defined in the absence of the index fossil foraminifer for the Aquitanian (Paragloborotalia kugleri) in the Central Paratethys (e.g. Báldi et al., 1999; Piller et al., 2007). The vertebrate fauna contains elements (e.g. the anthracothere Microbunodon minimum) that would rather support Latest Oligocene age (Rabi et al., 2018); however, the mixed nature of the fauna cannot discard the possibility that some of these remains were reworked from older beds.
In this study, we conducted rare earth element (REE), as well as oxygen and strontium isotope analyses to better constrain the depositional history, palaeoecology, and the age of the fossil fauna.
Taphonomical and sedimentological investigations of the vertebrate fossils (bones and teeth) are extremely useful to determine the depositional histories and environmental settings of vertebrate assemblages (e.g. Behrensmeyer, 1991; Botfalvai et al., 2015, 2017). Besides these traditional investigations, geochemical analyses of the fossils are also commonly applied (e.g. Anderson et al., 2007; Kocsis et al., 2020; Trueman, 1999, 2007). Bone fossilization can be viewed as the process of recrystallization of biogenic apatite to carbonate fluorapatite (francolite), and during this process many trace elements can be incorporated into the fossil bones (Keenan & Engel, 2017; Trueman & Tuross, 2002). Apatite has a strong affinity for the rare earth elements (REE), which are only sparsely present in body fluids, but soil and pore waters often contain somewhat larger concentrations of these elements (Trueman, 1999, 2007). When the bones are exposed postmortem to these fluids, the concentrations of REEs increase dramatically in a relatively short time during the fossilization; hence, the buried bones record fingerprints or signatures diagnostic of the burial environment (e.g. Trueman & Tuross, 2002; Trueman et al., 2006). Based on these listed features, the bones that recrystallized in different depositional settings inherit different trace element (TE) compositions, and thus, variations in the TE signatures within a bonebed can be used to infer post-depositional transport and mixing of vertebrate assemblages and to unravel the accumulation histories of such mixed faunas (Botfalvai et al., 2022; Kocsis et al., 2020, 2021; Trueman et al., 2004, 2006). Therefore, the fossil-rich, ecologically mixed vertebrate accumulation of the Máriahalom is an ideal site to test the remains with geochemical taphonomy. The incorporation of REEs into the bioapatite structure depends on the crystal size and original organic content of the investigated material. The uptake can be also viewed as a diffusive process from the external REE source of the pore fluid towards the internal parts of bones and teeth, and concentration gradients along specimens are often observed (e.g. Herwartz et al., 2013; Kohn & Moses, 2012; Millard & Hedge, 1996; Schroeter et al., 2022; Trueman et al., 2011). On the other hand, protracted REE uptake with time was also reported (Herwartz et al., 2011; Kocsis et al., 2010; Kowal-Linka et al., 2013) that may hinder palaeoenvironmental interpretation of the early diagenetic conditions.
Although the REE elements are almost entirely incorporated into the bioapatite [~ Ca5(PO4, CO3)3(OH, F, CO3)] during diagenesis, stable isotope compositions of the major anions (δ13CCO3, δ18OCO3, δ18OPO4) of fossil teeth and bones can still reflect ecological conditions and habitats of the vertebrates and, therefore are widely used in palaeoenvironmental and palaeoclimate studies (e.g. Kohn & Cerling, 2002 and reference therein). Oxygen isotope composition of ectotherm aquatic organisms can be linked to ambient temperature and the isotopic composition of environmental water (e.g. Kolodny et al., 1983; Lécuyer et al., 2013). In case of terrestrial mammals, carbon isotopic composition of their skeletal remains can reflect diet, while the oxygen isotope composition could be related to their body water via which the local climate can be traced (e.g. Bryant & Froelich, 1995; Fricke et al., 1998; Kocsis et al., 2014; Kohn & Cerling, 2002; Longinelli, 1984; Makarewicz & Pederzani, 2017; Tütken et al., 2006, 2013).
Strontium isotope ratios (87Sr/86Sr) of well-preserved marine calcareous and phosphatic fossils are often used to date marine sections via comparing the obtained values with the global Sr isotope reference curve (McArthur et al., 2001, 2020). The basis of this method lies in the fact that Sr has a long residence time in seawater (> 106 yrs), longer than that of oceanic turnover (103 yrs), which results in an overall unique Sr isotope composition for the open ocean (e.g. Burke et al., 1982; DePaolo & Ingram, 1985; Veizer, 1989). However, on a longer time scale, the 87Sr/86Sr of the ocean varied depending on the proportion of the Sr input into the ocean (i.e. higher ratios—continental crustal source, lower ratios—mantle-derived Sr). When marine organisms secret their shells or skeleton, they incorporate Sr into their biominerals without fractionation; hence, their remains can be used to trace open ocean origin and the age of their embedding sediment.
The Paratethys evolved as an epicontinental sea initially covering the present area between the Western Alps and Lake Aral. It was isolated from the Tethys Ocean during the Late Eocene–Early Oligocene due to the Alpine orogeny related to Africa’s northward movement and counterclockwise rotation resulting in the subduction of the European plate (Popov et al., 2004 and references therein). This caused the final disintegration of the Western Tethys Ocean and created the Mediterranean Sea at the western end of the Tethys, while the Paratethys Sea arose north of this tectonic belt. The connection between the two basins was restricted in certain times, which significantly limited faunal exchange leading to different fauna compositions.
The Paratethys can be separated into three palaeogeographic and geotectonic units namely Western- Central and Eastern Paratethys each recording a different environmental history (Piller et al., 2007). The Eastern Paratethys is the largest part and includes the present-day area of Black Sea, Caspian Sea, and Lake Aral basins, while the Western part comprises the Alpine Foreland Basins of France, Switzerland, and part of Germany and Upper Austria (Piller et al., 2007; Popov et al., 2004; and references therein). The Central Paratethys is situated between the western and eastern realms, and it consists of the Eastern Alpine—Pannon–Carpathian Basins (Fig. 1b). The former area of Máriahalom was situated in the Central Paratethys during the Late Paleogene–Early Neogene period (Piller et al., 2007; Popov et al., 2004; Fig. 1b).
Isolation and reopening of oceanic gateways to the Paratethys occurred repeatedly, allowing the development of distinct palaeobiological provinces characterized by endemic evolution and difficulties in global stratigraphic correlations.
Máriahalom is in the Transdanubia Range of Hungary, which is part of the Hungarian Paleogene Basin (HPB). The HPB was filled up with sediments from the Middle Eocene to Early Miocene interval forming a single extensive sedimentary cycle. During the Eocene and the earliest Oligocene, the transgression gradually progressed from SW to NE, characterized by intensive faunal exchange with the Mediterranean realm. During the Early Oligocene, large parts of the Transdanubian Range was uplifted, and denudation removed the top part of the Eocene sections and the entire Lower Oligocene deposits (Báldi, 1983; Kázmér et al., 2003; Telegdi-Roth, 1927), while restricted, anoxic shales (Tard Clay Formation) were deposited in the eastern (Fig. 1c), deeper part of the HPB basin. In this time, the seaway towards the Mediterranean and also the northern connection to the global marine system had been closed due to the Alpine-Carpathian orogeny and eustatic sea level drop (Miller et al., 2020).
From the later part of the Kiscellian (Late Rupelian; Tari et al., 1993), transgression allowed re-establishing marine connections towards the Mediterranean. Thereafter, a new shallow marine basin is formed with lagoonal and freshwater deposition towards the margins (Hárshegy Sandstone Formation) (Szederkényi et al., 2012; Sztanó et al., 1998). Due to gradual subsidence of the basin, shallow bathyal deposition prevailed, and the Kiscell Clay Formation accumulated (Báldi, 1983).
During the Late Oligocene–Early Miocene (Egerian stage), sediment input from the west increased and long-term normal regression resulted in the interfingering alluvial Csatka and the marginal marine to neritic Törökbálint formations (Korpás, 1981; Nagymarosy & Gyalog, 1997; Sztanó et al., 1998). The Törökbálint Formation represents deposits from brackish to normal marine conditions of which the brackish part is separated as the Mány Member (Korpás, 1981; Nagymarosy, 2012; see Fig. 1c). The Oligocene/Miocene transition is characterized by a regression trend (e.g. Széchényi Schlier Formation) and in many cases the Early Miocene denudation also affected the deeper Oligocene beds, resulting in reduced thickness of Paleogene strata.
Position and depositional environment of the Máriahalom site
The Máriahalom site is an abandoned sand-pit located 47 km north-west from Budapest (Hungary), between the villages of Úny and Máriahalom, within the Mány-Zsámbék Basin (part of the Transdanubian Range) of the HPB (Fig. 1a). The exposed section in the quarry is predominantly characterized by highly matured quartz sand (beach), sandy clay, and interbedded sandstone bodies. The exposed sediments are characterized by a predominantly brackish mollusc fauna (Potamides-Pirenella community, see detailed in Báldi 1967 and Báldi & Cságoly, 1975) that represents a transition between alluvial and normal marine conditions. It is interpreted as a product of lagoonal environments (Báldi, 1967; Nagymarosy & Gyalog, 1997; Nagymarosy, 2012), which was possibly connected to a delta plain environment (Szabó et al., 2017; Sztanó et al., 1998).
The vertebrate fossils studied here were found isolated and they have been collected from 2 to 5 m wide, 1–1.5-m–thick coquina-like accumulations situated in the uppermost part of the exposed section at the northeast wall of the quarry. This coquina-like, bonebed accumulation is composed almost entirely of transported, abraded, and mechanically sorted mollusc shells, where the amount of the siliciclastic sediments is subordinate compared to the fragmented bioclasts. In the bonebed, remains of shallow marine (sharks, rays, and bony fishes), freshwater (turtles, crocodiles, and mammals), and terrestrial (mammals) vertebrates are found together probably as a result of wash-in of skeletal elements from the shore into the marine depositional area due to wave action (Rabi et al., 2018; Szabó et al., 2017).
Based on the above explained sedimentological, taphonomical, and palaeontological evidence, the depositional environment is characterized by high energy conditions with tidal influence (e.g. Szabó et al., 2017). The bone-rich bed probably represents a channel fill deposit, which has been formed on a delta plain embayment or a tidal flat characterized by intense tidal influence (Szabó et al., 2017). In summary, the examined vertebrate assemblage was deposited in a transitional environment between the normal marine and terrestrial habitats, which was characterized by relatively low salinity and high energy conditions (Rabi et al., 2018; Szabó et al., 2017).
Age of the Máriahalom fauna
The above-mentioned sedimentological and palaeontological data indicate that the examined section at the Máriahalom site corresponds to the Mány Member of the Törökbálint Formation (Báldi, 1967; Nagymarosy & Gyalog, 1997; Nagymarosy, 2012; Szabó et al., 2017). Deposits of the Mány Member belong to the transitional zone between the marginal marine to neritic Törökbálint Sandstone Formation and the alluvial (deltaic) system of the Csatka Formation (Fig. 1c). The 300–500-m-thick Mány Member belongs to the Egerian Paratethys stage based on mollusc biostratigraphy derived from more than 50 boreholes (Báldi et al., 1999; Báldi & Cságoly, 1975; Nagymarosy & Gyalog, 1997; Piller et al., 2007). The Egerian chronostratigraphic stage is a local stage developed for the Central Paratethys Basin. The age of the basal part of the Egerian stage corresponds to the upper Chattian, while the upper part can be correlated with the lower Miocene (Aquitanian or Aquitanian / Burdigalian boundary) based on biostratigraphy and preliminary Sr isotope stratigraphy data (Báldi et al., 1999; Less et al., 2015; Piller et al., 2007; Rögl, 1998). However, the Oligocene/Miocene boundary is difficult to detect in the Egerian stage due to the absence of Aquitanian marine index fossils in the Central Paratethys region such as the Paragloborotalia kugleri (e.g. Báldi et al., 1999; Piller et al., 2007).
Despite the chronostratigraphic uncertainties of the Egerian stage mentioned above, the exposed section of the Mány Member at the Máriahalom site was interpreted and referred to as an Oligocene succession (Báldi & Cságoly, 1975; Rabi & Botfalvai, 2008; Rabi et al., 2018; Szabó et al., 2017) on the basis of the presumed stratigraphic position of the brackish mollusc fauna in the basin (Báldi & Cságoly, 1975; Piller et al., 2007), and the fossil terrestrial mammal Microbunodon minimum (Cuvier, 1822) (Rabi & Botfalvai, 2008; Rabi et al., 2018). However, the new 87Sr/86Sr data reported in this study suggest an earliest Miocene age instead (see below).
The invertebrate and vertebrate taxa from Máriahalom include normal marine, freshwater, and terrestrial elements (Table 1). All vertebrate remains consist of isolated teeth and mostly fragmentary bones.
The remarkably abundant and well-preserved mollusc fauna of Máriahalom has been classified as a Potamides-Pirenella community, possibly reflecting a brackish lagoon environment, although less common, normal marine taxa (e.g. Glycymeris latiradiata, Mytilus aquitanicus) are also present (Báldi & Cságoly, 1975; Janssen, 1982).
The fish assemblage represents a marine subtropical-tropical littoral community (Szabó et al., 2017). Based on the isolated elasmobranch and bony fish remains 8 sharks, 4 rays, and 7 teleosts were identified. The fauna is dominated by piscivorous taxa (odontaspids and carcharhinids); however, large, macropredatory sharks (e.g. Otodus) also occurred.
The reptilian material includes remains of turtles (trionychid and testudinoid), crocodilians, and squamata (Rabi & Botfalvai, 2008). Regarding mammals, despite the small sample size of the carnivoran remains, four separate taxa could be identified including the semi-aquatic basal mustelid Potamotherium valletoni, the small-sized, terrestrial basal mustelidean Amphictis sp. or Franconictis sp., another indeterminate basal mustelidean, and the medium-sized, terrestrial basal ursoid Pachycynodon boriei (Rabi et al., 2018). Other mammals such as Microbunodon minimum, sirenians, and medium-size herbivore mammals were also discovered (Rabi & Botfalvai, 2008).
Material and methods
Trace element analyses
From 2- to 4-mm-sized chips of bones and teeth were taken from 16 selected samples including remains of terrestrial, freshwater, and marine animals. The coverage of the different habitats within the mixed vertebrate fauna was important to check whether different taphonomic signatures could be detected from possible previous deposits. The chosen specimens, and the type and parts of the bones and teeth, are listed and illustrated in Additional file 3: Table S1 and Additional file 1: Figure S1. The specimens were embedded in epoxy resin and then polished for Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS). The ablation was carried out in a Helium atmosphere using spot sizes of 100 μm in diameter. Each specimen was ablated between 1 and 4 times. Trace element contents were determined using an Element XR single collector sector field ICP-MS interfaced to a RESOlution SE 193 nm excimer ablation system equipped with an S155 two-volume ablation cell. The analyses were carried out at the University of Lausanne, Switzerland. Our study focused on the rare earth elements; however, other trace elements were analysed too (see Additional file 3: Table S1). Standard reference material of NIST612 was used for external standardization, and as an internal standard 42Ca was analysed, and CaO values of 54 and 50 wt.% were used for enameloid/enamel and for dentine and bone, respectively (e.g. Kocsis et al., 2021). The analytical reproducibility was generally better than ± 5% SE. The obtained data allow relative comparison between samples and sites using element ratios or PAAS (Post Archean Australian Shale)-normalized REE patterns. The REEs are subdivided into light (LREE: La, Pr, Ce, Nd), middle (MREE: Eu, Gd, Tb, Dy), and heavy rare earth element (HREE: Er, Tm, Yb, Lu) groups. MREE* is defined by the average of LREE and HREE.
Stable isotope analyses
To test palaeoenvironmental conditions at the Máriahalom site, vertebrate taxa characterized by different ecology were chosen for stable isotope analyses. Four shark teeth (Araloselachus cuspidatus VER 2016.2743., Carcharoides catticus VER 2016.2741., and two odontaspids VER 2016.2744.), a cf. Diplocynodon sp. crocodilian tooth (VER 2016.3561.), a seacow rib (VER 2016.3562.), a carnivore (Potamotherium valletoni VER 2016.3565.), and two herbivore mammal teeth (Microbunodon minimum, VER 2016.3563. and VER 2016.3564.) were prepared for the measurements. The samples were cleaned in an ultrasonic bath; then enameloid, enamel, and in some cases, dentine and bone, were sampled with a microdrill. The powders were briefly leached in acetic acid buffered by Ca Acetate to pH 4.5 to remove any exogenous carbonate. A subset of the samples was prepared via separating the PO43− ion that precipitated as Ag3PO4 (e.g. Dettman et al., 2001; O'Neil et al., 1994) and then analysed for δ18O with high-temperature conversion elemental analyser (TC/EA, see Vennemann et al., 2002). Each sample ran as triplicates and the results were corrected to in-house Ag3PO4 standards that had better than ± 0.3 ‰ standard deviation. All these data are expressed relative to VSMOW (Vienna Standard Mean Ocean Water). NIST SRM 120c phosphorite standard material was run parallel with the samples and yielded δ18O values of 21.5 ± 0.2 ‰ (n = 4). Few calcareous shells were also measured for carbon and oxygen isotope compositions as a part of a screening method before SIS dating (see below). The analyses were done on a Gasbench II coupled to a Finnigan MAT Delta Plus XL mass spectrometer via reacting the samples with phosphoric acid (Spötl & Vennemann, 2003). The analytical precision for this method was better than ± 0.1‰ standard deviation. The stable isotope values are expressed in the δ-notation in case of the δ18OPO4 relative to VSMOW, while in the cases of the δ18OCO3 and δ13C values relative to VPDB (Vienna Pee Dee Belemnite, Additional file 4: Table S2). All these measurements were carried out at the stable isotope laboratory of Institute of Earth Surface Dynamics, University of Lausanne, Switzerland.
Strontium isotope ratios and related analyses
Different biominerals (aragonite, calcite, bioapatite) with different structures (shark tooth dentine vs enameloid) were selected for Sr isotope analyses. Bioapatite is represented by two shark tooth enameloid and one root, aragonitic shells by a Glycymeris bivalve and a Nerineidae gastropod, and lastly calcite shell by an Ostrea bivalve (Table 2). Some of the samples were further screened with different methods and the results are summarized in Table 2. First the samples were powdered, and prior to strontium isotope preparations, the three calcareous specimens were tested for mineralogical composition by X-ray powder diffraction using a Bruker D8 Advance powder diffractometer with parallel beam, 2θ-θ geometry equipped with LynxEye© 1D detector. The measuring parameters were step-scanning at 0.01° 2θ intervals and counting time of 35.4 s (0.2). CuKα radiation at 40 kV and 40 mA was used. The measurement range was 5–70° 2θ. The identification of minerals was achieved using the Diffrac EVA software by comparison of the X-ray diffraction pattern from the sample with the International Centre for Diffraction Data PDF-2, release 2009 database. The quantitative data were obtained using TOPAS software providing us a standardless quantitative analysis (based on Rietveld method). The measurements were made at MOL Laboratories in Budapest, Hungary. The obtained diffractograms are provided in Additional file 2: Figure S2 document. Sub-samples were also run for stable isotope analyses as described above (see also Table 2).
The samples were then further handled at the Department of Chemical and Geological Sciences of the University of Modena and Reggio Emilia (UNIMORE), Italy. They were dissolved, and sub-solutions of the calcareous samples were measured for concentration of Mg, Sr, Mn, and Fe on a Perkin Elmer Optima 4200 DV ICP-OES. The supernatant was diluted to 4% w/w HNO3 for the analyses, while an aliquot of the sample was further diluted to measure Ca concentration as well. The ICP-OES was externally calibrated with multielement calibration standards in the concentration range from 1 ppb to 10 ppm for all the elements. Precisions were typically better than 5% RSD (relative standard deviation) for Ca, Mg, Sr, and Fe and better than 20% for Mn. The data are expressed in ratios relative to Ca in mmol/mol (Table 2). The rest of the solution is then processed using standard cation exchange to separate strontium with Eichrom Sr-spec resin filled columns. The Sr isotopic ratios were analysed via a double-focusing MC-ICPMS with a forward Nier–Johnson geometry (Thermo Fisher Scientific, NeptuneTM). All the data were adjusted to an 86Sr/88Sr value of 0.1194 as fractionation correction. The samples were run together with several NIST SRM 987 standards, and their 2 standard deviations were used to correct for instrumental bias to a value of 0.710248 (McArthur et al., 2001). Repeated analyses of the NIST SRM 987 yielded external reproducibility (2SD) of 18 ppm (n = 14). The 87Sr/86Sr ratios of the samples then were compared to the look-up tables of McArthur et al. (2020), which is tied to the Geological Time Scale 2020 (Gradstein et al., 2020), and were used to derive numerical ages. The errors related to the analyses (internal and external reproducibilities) and the error associated with the Sr evolution curve (i.e. 95% confidence interval) were considered in the age calculations.
Rare earth element composition
Sixteen vertebrate specimens were analysed with multiple in situ laser spots (n = 41). The data were scrutinized and then averaged relating to the specimens, their different types of material (e.g. enamel vs dentine), and their PAAS-normalized REE patterns, resulting in 29 final data points (Fig. 2 and Additional file 3: Table S1). The total REE contents range from 1.3 to 10,045 ppm (mean = 1997 ppm; median = 1062 ppm). The lowest value is from a mammal tooth dentine (M16), while the highest REE content came from a crocodile scute (M12). Generally, the denser and higher mineralized material such as tooth enameloid/enamel yielded low REE concentration, and/or the more internal part of bone and dentine within the specimens. Another observation is that the lighter coloured bones show relatively higher mean REE content than the darker ones (e.g. crocodile scute M12 and M11; turtle plates M8, M9, and M7) (Additional file 1: Figure S1). In terms of taxa, the overall shark tooth data (M1-M3) have a lower REE content compared to bones and teeth of other vertebrates; otherwise, there is no significant difference in REE content between the terrestrial, freshwater, and marine remains.
The PAAS-normalized REE patterns show two endmembers, a middle REE-enriched pattern and a LREE-depleted ones (Fig. 2). The latter group has in general lower REE content. The spread of the data on an MREE/MREE* versus LREE/HREE diagram further emphasizes the REE distribution in the samples between these two endmembers. In addition, the data have larger variation along the MREE compared to LREE/HREE (Fig. 2). It must be pointed out that there are no taxonomic differences related to the REE patterns, or REE element ratios, i.e. the terrestrial (e.g. Microbunodon minimum), marine (e.g. sharks), or freshwater (e.g. turtles) groups are all along the same data spread. The apparent variation is rather related to where and in which material the REE concentrations derived from. For example, the ray tooth (M4—Fig. 2A—blue-edge triangle) yielded MREE-enriched pattern in the external part of the tooth, while the internal part of root has the LREE-depleted pattern.
Stable isotope compositions
Shark tooth enameloid yielded a range of δ18OPO4 from 19.5 to 21.9 ‰ (n = 4) with the highest value coming from the species Carcharoides catticus (Additional file 4: Table S2). The single dentine analysis from the Araloselachus tooth shows a much lower value of 17.8 ‰ compared to the enameloid. The crocodilian tooth has a similar pattern in showing higher isotopic value in the enamel (19.3 ‰) than in the dentine (18.5‰). An identical trend is apparent for the terrestrial mammal teeth as well where the enamel δ18OPO4 values are higher than those in the dentine. The upper and lower molars of Microbunodon yielded values of 17.4 and 16.3 ‰ in the enamel, and 15.9 and 15.4 ‰ in the dentine, respectively. In the case of the carnivore mammal (Potamotherium sp.), the difference between the two tooth structures is more subtle with δ18OPO4 of 15.7 ‰ in the enamel and 15.6 ‰ in the dentine. The lowest δ18OPO4 value comes from the seacow ribs with a value of 15.3 ‰. All the isotope data are plotted relative to environmental water isotope compositions (δ18OH2O), which were calculated for the different taxonomic groups: fish (Lécuyer et al., 2013), crocodilians (Amiot et al., 2007), and mammals (Kohn & Cerling, 2002; Tütken, 2003) (Fig. 3).
Strontium isotope ratios and related screening methods
Three bioapatite (shark teeth) and three calcareous mollusc shells were analysed for Sr isotope ratios. Prior to this analyses the calcareous remains were tested for mineralogy, trace element, and stable isotope compositions. X-ray diffraction measurement confirmed that the calcareous remains preserved most of their original mineralogy (Table 2, Additional file 2: Figure S2), which is particularly important for the aragonitic remains (G1 and N1) as these tend to recrystalize to calcite in the diagenetic environment. The Nerineidae gastropoda is 99% of aragonite, while the Glycymeris bivalve besides 92% of aragonite yielded 8% of calcite. These aragonitic molluscs yielded higher δ13C and δ18O than the dominantly calcitic oyster shell (Table 2). The Mg/Ca and Mn/Ca ratios are higher, while the Sr/Ca ratio is lower in the oyster specimens, compared to aragonitic shells. The Fe/Ca ratio is highest in the gastropod shell (N1) and lowest in the Glycymeris bivalve (G1). All these data are summarized in Table 2.
The 87Sr/86Sr ratio ranges from 0.708189 to 0.708898 (n = 6), the lowest ratio is measured in the oyster shell (O2), while the highest from the root of a shark tooth (SR2). The rest of the samples are rather confined to a relatively narrower range of 0.708364 ± 0.000029 (n = 4), though one of the shark teeth has somewhat higher 87Sr/86Sr than the rest of the three analyses (Fig. 4). Taking the average of these three specimens and considering its 2SD error (0.708350 ± 0.00022), an age estimates of 21.4 ± 0.5 Ma can be obtained (Aquitanian). However, the two extreme values would correspond to 24.2 Ma (O2—Chattian) and 9.6 Ma (SR2—Tortonian), respectively (Fig. 4). It is noted that the internal error of the individual Sr isotope analyses is smaller than that of the external error derived from repeated analyses of the NIST SRM 987 standard (18 ppm, 2SD, n = 14). Therefore, this higher, external error range was used to calulate the individual Sr isotope ages (Table 2).
Rare earth element taphonomy
Rare earth elements are almost entirely of early diagenetic origin in fossil vertebrate remains (e.g. Elderfield & Pagett, 1986; Schroeter et al., 2022; Trueman, 1999, 2007; Trueman & Tuross, 2002; Trueman et al., 2006); therefore, their REE content and their distribution within the remains can be linked to depositional conditions. The REE content of the burial environment can vary based on the available REE related to local geology, sedimentology, and hydrology, while their distribution often related to redox conditions (e.g. Haley et al., 2004; Kocsis et al., 2016; Reynard et al., 1999). Therefore, remains fossilized in different environments and under different conditions (e.g. redox, terrestrial vs. marine) could inherit different REE compositions. A major implication of this is to test mixed vertebrate assemblages whether they contain fossils reworked from older and/or from different fossilization setting (e.g. Botfalvai et al, 2022; Kocsis et al., 2020, 2021; Trueman, 1999; Trueman et al., 2003). The fact that the investigated different materials from Máriahalom (e.g. bone, dentine vs enamel/enameloid) yielded similar REE distribution trends with decreasing REE concentration interiorly would partially support no, or if at all, any mixing of different REE sources, and would suggest contemporaneous REE uptake (e.g. Kohn, 2008; Trueman et al., 2011; Ullmann et al., 2020).
The relatively low REE content in the shark teeth enameloid at Máriahalom is probably explained by their inherently denser structure raised by Harrell and Pérez-Huerta (2015), Kohn (2008), and Botfalvai et al. (2022). This could relate to their substantial amounts of fluorapatite, which is composed of larger crystals, and is more stable than the carbonate hydroxylapatite found in bone tissue. Therefore, these are less likely to recrystallize during fossilization and thus incorporate less REE. The low REE concentration in the thick mammal tooth enamel could also be related to the larger crystals, and originally lower organic content than that of dentine or bone. In case of larger remains, the internal parts within the bone cortex and teeth may not have access to pore fluid REE source, hence is their lower REE concentrations (e.g. sheltering effects of enamel for dentine). Similar, variable REE contents and different REE distribution were reported from single bones (e.g. Decrée et al., 2018).
The PAAS-normalized REE patterns and REE ratios (Fig. 3) show a trend between a middle REE-enriched and light REE-depleted patterns. Importantly, both patterns can occur in the same specimens. The LREE-depleted patterns mostly occur in the internal part of the samples, and in general, they have lower REE concentration. Therefore, these patterns are best explained by partial REE uptake driven by the limited access to REE, and by different diffusion/absorption rates along the REE series (e.g. Herwartz et al., 2011; Trueman et al., 2011) relating to their decreasing ion radii with increasing mass (i.e. lanthanide contraction). This latter process results in more effective adsorption of the LREE and MREE at the edge of the fossils during permineralization, while HREE is less likely scavenged, and it can be relatively more enriched in the interior part of the bones (e.g. Herwartz et al., 2013; Ullmann et al., 2021). Such trends have been reported from many environmental settings (e.g. Botfalvai et al., 2021, 2022; Herwartz et al., 2013; Kocsis et al., 2020; Ullmann et al., 2020). On the other hand, the MREE-enriched patterns would reflect the distribution of external REE sources related to early diagenetic pore fluids. The REE signature of natural water in the transitional zone between the marine and the terrestrial environments (such as estuaries) is highly variable, but usually is intermediate between the REE signatures of river and sea waters (Elderfield et al., 1999; Patrick et al., 2004). The LREE and MREE are preferentially adsorbed onto particle surfaces, hence in case of high sediment load in the coastal region could result in MREE-enriched patterns in vertebrate fossils such as observed in the Máriahalom assemblage (e.g. Elderfield et al., 1999; Patrick et al., 2004; Trueman et al., 2006). MREE-enriched patterns are sometimes also linked to low redox conditions and Fe redox recycling (Haley et al., 2004), and the quick sedimentation rate at the Máriahalom site may also have induced low redox conditions in the early diagenetic environment.
The similar distribution patterns of the normalized REE data from the bones and teeth of the marine, terrestrial, and freshwater animals indicate that all the investigated remains here were fossilized in similar diagenetic environments and had comparable REE uptake history. The fact that the Microbunodon minimum remains (both of teeth and bones) and the other measured fossils are very similar both in REE content and distributions suggest a contemporaneous fossilization for the entire vertebrate assemblage. Another implication is that even if evident mixing occurred, large-scale reworking from strata of significantly different ages can be excluded.
The isotope composition of teeth and bones (δ18OPO4) of the examined taxa is closely related to environmental water isotope composition (δ18OH2O) such as to ambient water for aquatic organisms and to consumed water for terrestrial animals (e.g. Amiot et al., 2007; Kohn & Cerling, 2002). In the case of fishes, ambient water temperature is also an important factor controlling the δ18OPO4 values (Kolodny et al., 1983; Longinelli & Nuti, 1973).
The isotope composition of environmental water can vary a lot (e.g. Dansgaard, 1964). The average isotope composition of seawater is 0 ‰ (i.e. standardized to mean ocean water—VSMOW). However, locally, sea surface water can be more enriched in the heavy isotopes due to evaporation (positive δ18OH2O), or it can be depleted in 18O due to freshwater input (negative δ18OH2O). The negative δ18O of freshwater is a result of successive evaporation and condensation processes of water vapour (Rayleigh fractionation), when meteoric water (i.e. precipitation) becomes more and more depleted in the heavy isotope (18O) than its original source (e.g. seawater). This depletion is further enhanced, for example, by altitude, latitude, and distance from the ocean. On the other hand, the average isotopic composition of seawater fluctuated mainly due to climatic factors such as temperature and the amount of ice in the Polar Regions in the past. Generally, for the Cenozoic the average δ18OH2O of seawater varied between a greenhouse and icehouse endmember values of about −1 ‰ and 0.5 ‰, respectively (e.g. Lear et al., 2000).
Two major observations can be drawn from the phosphate oxygen isotope data from Máriahalom: (1) there is a trend towards lower δ18OPO4 values from enameloid/enamel to dentine and bone samples, and (2) there is a decrease in the enameloid/enamel isotopic values from shark teeth to the successively lower values of crocodilians and mammals (Fig. 3).
The consistent negative offset between enameloid/enamel versus dentine and the very low isotope value of the sirenia rib bone most probably reflect at least partial diagenetic overprint by low isotopic composition fluids (i.e. brackish/freshwater pore fluids). Generally, the bioapatite in dentine and bone have smaller crystallite size and higher organic content than enameloid/enamel. These make them more prone to alteration, meaning higher susceptibility to secondary apatite formation in the pore spaces of the remains (e.g. Trueman & Tuross, 2002). In this case, some portion of the apatite would reflect the isotopic composition of the pore fluid from which it is precipitated. The fauna at the Máriahalom site clearly shows freshwater influence in the region (i.e. terrestrial mammal fossils and brackish gastropod community; Báldi, 1967; Báldi & Cságoly, 1975; Báldi, 1983; Szabó et al., 2017), and probably after deposition fresh and/or brackish pore fluid dominated the burial environment, which eventually could have enhanced the above-described diagenetic process.
The quantities of the original and secondary apatite in the vertebrate remains are difficult to assess. In case of sharks, as they replace their teeth rapidly, enameloid and dentine of the same tooth are expected to be similar in their isotopic composition. On the other hand, mammal dentine can mineralize at different stages than enamel, and in rare cases, it may remodel during the life of the animal (e.g. during healing process) (Koenigswald, 2020); hence, different δ18OPO4 values between enamel and dentine would not be exceptional. Regarding the sirenia rib bone, the very low δ18OPO4 value is too low for a predominantly near-shore marine mammal and the obtained value of 15.3 ‰ would indicate rather freshwater habitat (Clementz & Sewall, 2011; Tütken, 2003). However, even if trichechine sea cows (manatees) include freshwater taxa (Suarez et al., 2021), the data presented here from the bone and dentine samples are considered partially altered. Conversely, the data derived from the more resistant enameloid/enamel can be further discussed in terms of ecological conditions.
The highest δ18OPO4 values are derived from the shark teeth (Fig. 3). The isotopic composition of fish teeth is the function of isotopic composition of environmental water and ambient temperature (Kolodny et al., 1983; Lécuyer et al., 2013; Longinelli & Nuti, 1973). When the Máriahalom shark data are plotted in the space of water and phosphate isotopic compositions with calculated isotherms (Lécuyer et al., 2013) at a Late Oligocene seawater isotopic composition (Fig. 3, −0.3 ‰, Lear et al., 2000), the observed temperature range (Fig. 3) is compatible with the inferred habitat preference of the given shark taxa (e.g. Compagno et al., 2005). As mentioned earlier, seawater isotope composition can vary due to evaporation or freshwater input especially in coastal regions. More freshwater influenced milieu would lower the δ18OPO4 values, while habitat in evaporative lagoons / or colder, deep water could result in higher δ18OPO4 (Fig. 3). However, variation in the isotope data among the shark teeth may not only be explained by these processes depending on where the sharks lived, but also may relate to traces of anomalous metabolic oxygen (Feng et al., 2022).
Regarding crocodilians, Amiot et al. (2007) reported a relationship between their bioapatite and ambient water isotopic composition within a precision of about ± 2 ‰: δ18OH2O = 0.82 × δ18OPO4—19.13. Using this correlation with the analysis of the cf. Diplocynodon crocodilian tooth enamel from Máriahalom an environmental water isotopic composition of −3.3 ‰ can be calculated, which indicates a brackish palaeohabitat of this animal. Most of the remains of Diplocynodon frequently collected from sediments deposited in freshwater environment (e.g. Buscalioni et al., 1992; Piras & Buscalioni, 2006; Delfino & Smith, 2009; Martin, 2010; Martin et al., 2014; Luján et al., 2019; Rio et al., 2019; Chroust et al., 2021 and references therein); however, Diplocynodon remains were also found at localities, which originated in a coastal lagoon characterized by salinity fluctuations (e.g. Monteviale, Italy: Macaluso et al., 2019; Pandolfi et al., 2016) or in normal marine conditions (Viştea Limestone, Romania; Sabău et al., 2021). It is currently difficult to decide whether Diplocynodon was able to tolerate saline water or not (e.g. Brochu, 2001), but our data and the cited studies probably suggest this crocodile could have tolerated at least the brackish conditions.
Contrary to fishes and crocodilians, mammals maintain constant body temperature, and therefore, the isotope composition of their teeth reflects body fluid isotopic composition that is also influenced by the metabolism of the animal (e.g. Bryant & Froelich, 1995; Kohn & Cerling, 2002). Apart from arid-adapted mammals, most large terrestrial herbivores show a strong correlation between local water (i.e. drinking water) and bioapatite isotope compositions (e.g. δ18OH2O = 1.11 × δ18OPO4 −25.6; Kohn & Cerling, 2002). Using only the enamel results of the Microbunodon teeth, environmental water of −6.9 ± 0.9 ‰ can be obtained. Therefore, the low δ18OPO4 values in the Máriahalom mammal teeth indicate that the body fluid of these animals was influenced by this depleted water source (e.g. spring, river, lake) that was used as drinking water and/or was obtained via consumption of plants influenced by low δ18OH2O precipitation. Generally, Microbunodon lived in highly forested and marshy habitat (Boisserie, 2007; Lihoreau & Ducrocq, 2007; Tsubamoto et al., 2012), which could agree with the oxygen isotope data. Regarding the semi-aquatic carnivore mammal Potamotherium valletoni, the low δ18OPO4 values (as seen for other aquatic mammals; cetacean: Roe et al., 1998; sirenian: Newsome et al., 2010) are compatible with an already inferred freshwater habitat based on depositional environments of Western European occurrences of this taxon (e.g. Savage, 1957). This is of relevance because comprehensive phylogenies find Potamotherium valletoni as a stem-pinniped, and predict prolonged freshwater phases prior to marine adaptation seen in the crown group based on taphonomic data (Paterson et al., 2020). Our data lend independent support to this hypothesis, and P. valletoni may have not utilized the available normal marine habitats at Máriahalom.
Age of the Máriahalom site
The 87Sr/86Sr ratio of the Phanerozoic open ocean was consistent at any geological time all around the world owing to the long resident time of Sr in seawater, but the ratio varied with time as a function of gradual change in Sr influx from different Sr sources (e.g. DePaolo & Ingram, 1985; Frank, 2002; Veizer, 1989). Marine organisms, when secreting their calcareous or phosphatic hard tissues, record the actual Sr isotope ratio of the seawater; therefore, well-preserved fossils can be used to date marine sedimentary sequences via comparing the data with the Sr isotope evolution curve of the open ocean (SIS—Strontium Isotope Stratigraphy e.g. Hodell et al., 1991; Koepnick et al., 1985; McArthur et al., 2001, 2020; Veizer et al., 1997). A prerequisite is that the biominerals of the organisms are well-preserved and did not go through significant diagenetic alteration. Calcareous remains are commonly tested for original mineralogy (XRD), original shell microstructure (SEM), stable isotope, and minor/trace element compositions to ensure reliable Sr isotope ratio for age determination. Bioapatite, especially the dense crystals of tooth enameloid of fishes, has been also successfully used to date marine sequences (e.g. Harrell et al., 2016; Ingram, 1995; Kocsis et al., 2013). Nevertheless, as explained in the previous chapter, bioapatite readily uptakes REE and other trace elements during early diagenesis, and depending on the pore fluid composition in terms of Sr content, as well as Sr ratio, the fossils might be altered during this process (e.g. Martin & Scher, 2004).
Regarding the Máriahalom data, the calcareous fossils yielded original shell mineralogy. The aragonitic shells of the bivalve (G1) and gastropod (N1) have stable isotope and trace/minor element compositions that are for fully marine organisms; meanwhile, the geochemical data of the calcite oyster shell have lower δ13C, δ18O, Sr/Ca, and higher Mg/Ca ratios. This latter could indicate brackish habitat that is well known for certain oyster taxa (e.g. Kirby, 2001; Kirby et al., 1998). The relatively high Mn/Ca ratio may point towards alteration; however, in view of the brackish estuarine habitat presumed for the depositional environment of the Máriahalom site (Báldi & Cságoly, 1975), this could also be linked to in vivo incorporation. The minor presence of quartz from the XRD analyses of the oyster shell (Table 2, Additional file 2: Figure S2) may represent small grains that incorporated during shell growth in the high energy environment.
From our six Sr isotope analyses, three samples (the two aragonite shells and one shark tooth enameloid) yielded consistent Sr isotope ratios from which an average SIS age of 21.4 ± 0.5 Ma can be obtained (Aquitanian, Lower Miocene). Another shark enameloid yielded somewhat higher Sr isotope ratios (i.e. younger age of 20.6 Ma—Fig. 5), that may reflect minor alteration of its 87Sr/86Sr ratio. The sampled shark tooth root clearly reflects strong alteration with a derived false Tortonian age (which can be completely excluded due to the stratigraphical position of the site). These agree well with the earlier-mentioned observation from the REE and stable isotope studies as the more porous vertebrate materials (i.e. tooth root) were affected by more enhanced pore fluid exchange and alteration. The lowest Sr ratio comes from the calcite oyster shell corresponding to an SIS age of 24.2 Ma (Chattian, Upper Oligocene). In view of the other Sr isotope data, such old age could be explained by reworked origin of this shell from older beds; however, this would contradict with the interpretation of the REE data derived from the vertebrates. Alternatively, the lower Sr content and stable isotopic ratios could also indicate brackish conditions and, in part, freshwater Sr source that may have been influenced by somewhat older sediments that could have been exposed in the hinterland and drained into the basin (e.g. the clastic Csatka Fm—see Fig. 1—it contains Mesozoic and Eocene carbonate pebbles as well see Báldi, 1983).
The previously suggested age of the Máriahalom fauna was Egerian (Late Oligocene—Early Miocene), and due to the lack of global biostratigraphic markers, the actual Oligocene—Miocene boundary cannot be determined in the Paratethys. Despite this chronostratigraphic uncertainty of the Egerian stage, the exposed section of the Mány Member at the Máriahalom site was interpreted and reported as an Upper Oligocene succession (Báldi & Cságoly, 1975; Rabi & Botfalvai, 2008; Rabi et al., 2018; Szabó et al., 2017). Two main arguments are often brought up supporting the Oligocene age:
The Máriahalom site belongs to the lower part of “Mány Formation”, where the top of the Egerian stage may have been missing due to an erosional unconformity (Báldi & Cságoly, 1975; Piller et al., 2007). This is based on the brackish mollusc fauna of the site that is normally common in the lower part of the “Mány Formation”, while the upper part of the member is characterized by a sub-littoral marine fauna where the brackish elements are totally absent (Báldi & Cságoly, 1975). However, more recent lithostratigraphic work considers the “Mány Formation” as an interfingering unit of the normal marine Törökbálint Formation (Mány Member; Nagymaros et al., 2023).
The anthracothere mammal Microbunodon minimum (Cuvier, 1822) from Máriahalom site (Rabi & Botfalvai, 2008; Rabi et al., 2018) constrains the age to the MP28–MP30 mammal zone (European Mammal Paleogene Reference Levels) of the Upper Oligocene (Late Chattian; Lihoreau et al., 2004; Mennecart, 2015; Russell et al., 1982; Scherler et al., 2013).
Based on our new Sr isotope analyses we suggest an Aquitanian age of 21.4 ± 0.5 Ma for the sediments of the Máriahalom site. Our REE analysis suggests a common diagenetic history of Microbunodon minimum with that of other fossils from the locality and contemporaneous marine and terrestrial taxa. So far, the youngest occurrence of Microbunodon minimum has been reported from MP30 mammal level (~ 22.9 Ma see Mennecart, 2015; Scherler et al., 2013); hence, assuming that the terrestrial mammals and sharks from Máriahalom are synchronous, our data would stretch the temporal range of this taxon with at least 1–1.5 million years. This statement, however, seems to be of great importance, since the European occurrence of Microbunodon minimum can be dated to a restricted time interval (MP28–MP30 zones) of major faunal renewal in Europe. The MP28 marked an important immigration event in Western Europe resulting in progressive replacement of the Arvernian, hoofed mammals towards the establishment of the ‘‘classical’’ Agenian fauna (see details in Mennecart, 2015; Scherler et al., 2013). This transition started with MP28 and involved the appearance of Microbunodon in Europe. Due to its high significance in the reorganization of European ungulate communities, Scherler et al. (2013) proposed the name “Microbunodon event” to designate the starting datum of this important period. Microbunodon minimum is considered to have a short-term occurrence in Western Europe (Austria, France, Germany, and Switzerland) from MP28 to MP30 (Lihoreau et al., 2004; Scherler et al., 2013). The emergence of M. minimum in Europe seems to be linked to the global climatic event, named as Late Oligocene Warming (Zachos et al., 2001). The disappearance of the Microbunodon minimum occurred in the MP30 zone based on the Western European record (Scherler et al., 2013), which may be linked to another global climatic change, named as Mi-1 Glaciation Event. Mi-1 Glaciation Event can be characterized by decrease in temperature and humidity, and the palaeoenvironment became more mosaic like, represented by open landscapes and closed woodlands (Costeur et al., 2012; Mennecart, 2015). However, despite the comprehensive synthesis works published in the last decade, the timing and the causes of this short-term occurrence of Microbunodon minimum in Europe are still not well understood, and the fossil record is mainly based on remains of Western Europe. In any case, the dates of appearance and disappearance of this taxon are well-correlated with the mentioned two global climate changes, so the associated changes in vegetation can certainly provide a reasonable explanation for the brief occurrence of this animal, at least in Western Europe. The opening of the environment with less forest during the Latest Oligocene detected at the Western European localities (e.g. Becker et al., 2009; Mennecart, 2015; Mennecart et al., 2012; Scherler et al., 2013) could well have affected M. minimum, to have lived in a forested environment (Lihoreau & Ducrocq, 2007). Nevertheless, these environmental conditions were not uniform throughout the whole continent of Europe. Therefore, it cannot be excluded that vegetation conditions to the east or south of the Western European localities may have allowed this species to survive after the Oligocene–Miocene transition. Several tectonic events and geographical changes (e.g. Alpine orogeny) occur throughout the European Late Oligocene and Early Miocene geological history, which has created many geographical barriers (e.g. European Cainozoic Rift System or the different sub-basins of Paratethys) among different parts of Europe, where the different areas could have hosted slightly different vegetation and mammalian fauna compositions (Costeur & Legendre, 2008; Mennecart, 2015). Even between well-studied Western European localities, there are significant differences in the composition of mammal faunas during the Oligocene–Miocene transition (Costeur & Legendre, 2008; Costeur et al., 2012; Mennecart, 2015). Western European sites (especially vertebrate localities of France, Germany, and Switzerland) with their high diversity and several fossil-rich horizons provide most of the material for the establishment of the mammalian levels during the Oligocene and Miocene periods, which is understandable, since the eastern European material is less studied, and the age of the sites is often unclear (e.g. Rabi et al., 2018; Scherler et al., 2013). Our new 87Sr/86Sr data for Máriahalom mammal locality suggest that Microbunodon minimum may have survived till the MN 1–2 mammal zone (European Mammal Neogene Reference Levels) in the Central Paratethys region. This area was characterized by various combinations of mesophilous (zonal vegetation), riparian as well as swamp (intrazonal vegetation) vegetation types under humid subtropical climate during the Egerian (Erdei & Bruch, 2004; Hably, 1988, 1994 and references therein), which palaeoenvironment could have been a refuge area for M. minimum.
An alternative explanation is that the upper age limit of MP30 may not be precisely constrained (i.e. younger than ~ 22.9 Ma of Mennecart, 2015 and Scherler et al., 2013) since it is generally problematic to correlate terrestrial faunas (corresponding to temporally shorter levels) with better-dated marine faunas (temporally longer zones).
Besides Microbunodon minimum, four other mammalian taxa were described from Máriahalom including the semi-aquatic basal mustelid Potamotherium valletoni, the small-sized, terrestrial basal mustelidan Amphictis sp. or Franconictis sp., another indeterminate basal mustelidan, and the medium-sized, terrestrial basal ursoid Pachycynodon boriei (Rabi et al., 2018). Based on the fossil record of Potamotherium valletoni reported from France, Western Germany, and Switzerland, this taxon was present in Europe between MP28 and MN2a (Mödden & Wolsan, 1993; Morlo, 1996; Mörs & von Königswald, 2000); thus, our age data (MN1–2) for the Máriahalom site do not contradict with the presence of this taxon. Both Amphictis and Franconictis survive the Oligocene–Miocene transition and they were present in Europe until MN5 and MN3, respectively (Lucas et al., 1988; Mörs, 2002; Mörs et al., 2000; Nagel, 2003). On the other hand, the Pachycynodon boriei described from Máriahalom is perhaps problematic, since well-dated occurrences of this taxon have so far only been reported from the Early Oligocene (MP22) from France (Peigné et al., 2014; Rabi et al., 2018; Vianey–Liaud & Schmid, 2009). Rabi et al. (2018) suggested “the P. boriei material from Máriahalom extends the temporal distribution of this species to the late Oligocene (MP28–30)”, but in the view of our Sr isotope data this could be further extended into the earliest Miocene. It should be noted, however, that the genus Pachycynodon is restricted to the Oligocene (MP22), but in this case too, all the European data are from Western Europe (Peigné et al., 2014; Vianey–Liaud & Schmid, 2009).
The fossil vertebrate site of Máriahalom is investigated here with different geochemical approaches. The REE chemistry of the vertebrate remains points to alike early diagenetic conditions and REE source in the burial environment. Consequently, even if the fauna is mixed, significantly older reworked fossils cannot be expected. The δ18OPO4 data analysed on shark, crocodile, and land mammal teeth reflect marine, brackish/freshwater and terrestrial habitats, and suggest freshwater preference for the stem-pinniped Potamotherium valletoni. The 87Sr/86Sr data derived from well-preserved calcareous marine remains and shark teeth enameloid suggest an Aquitanian age (21.4 ± 0.5 Ma) for the sediments of the Máriahalom site, that is younger than the previously reported Late Oligocene age. In the view of this new age estimate, the mammal taxa of Microbunodon minimum and Pachycynodon boriei may have survived in the Central Paratethys region after the Oligocene–Miocene boundary and was present up till the MN1–2 biostratigraphic level. In the future, with the availability of additional age data, the temporal distributions of mammalian species in East-Central Europe areas will be further determined, which would provide a better understanding of the temporal and geographic changes in the mammalian faunas of Europe.
Availability of data and materials
All of the investigated fossils are housed at the palaeontological collections of the Hungarian Natural History Museum in Budapest (NHMUS). The geochemical data are provided in the Supplementary Table files.
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The support of the János Bolyai Research Scholarship of the Hungarian Academy of Sciences to G.B and the ELKH-MTM-ELTE Paleo Contribution [no. 374] are much appreciated. The authors are grateful for the constructive comments of Thomas Tütken and Daniel Herwartz on an early version of the manuscript.
Open access funding provided by University of Lausanne. This research was supported by the Hungarian National Research, Development, and Innovation Office (project NKFIH/OTKA PD 131557) to G.B.
The authors declare that they have no competing interests.
Handling editor: Thomas Martin.
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Specimens used for the rare earth element analyses.
X-ray diffraction results from the calcareous remains involved in the Sr isotope analyses (N1, G1, and O2, see Table 2).
PAAS-normalized rare earth element data derived from sixteen Máriahalom vertebrates remains.
Oxygen isotope composition of the investigated vertebrate remains from the Máriahalom site.
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Kocsis, L., Rabi, M., Ulianov, A. et al. Geochemical investigation of the mixed Máriahalom vertebrate fauna at the Paleogene–Neogene boundary in the Central Paratethys: environmental conditions and age constrain. Swiss J Palaeontol 142, 17 (2023). https://doi.org/10.1186/s13358-023-00281-7
- Stable isotopes
- Rare earth element taphonomy
- Strontium isotope stratigraphy
- Mammal biostratigraphy